CAT Practice 6

Hello everybody!

It’s almost the end of the semester, we have two more weeks of lectures and tests before we have one week of revision and then… exams! ><

Any who, today I will be doing a post for you all to practice on reading some patient and donor cards. As always, the answers will be at the bottom of the post 🙂

Oh and the featured image of this post is what we do in our lab! I love how this specialisation requires order, neat and structured work. Cleanliness is my jam.

ABO + Rh D (Patients)

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Tran Song Lum

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4360507

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4360513

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4079107

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4360532

  • What are their ABO groups?

(with regards to Tran Song Lum, his serum was showing fibrin clots as it was an old serum sample from the university and is not actually an anomaly)

Antibody Screen + Antibody Panel

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Tran Song Lum / Control

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4079107 / 4360532

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4360507 / 4360513

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Antibody ID Panel

  • What antibody does the patient have based on his panel? (You can find an empty ID panel to work this out on here)

Phenotyping Donors and Patient

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Tran Song Lum

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Donor 1

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Donor 2

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Donor 4

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Donor 5

  • What are the phenotypes of the patient and donors?
  • What is the patients Rh genotype (either in Fisher-Race or Wiener) ?
  • Is Tran Song Lum’s antibody a result of an allo-antibody or an auto-antibody?
  • Based on all the results gathered so far, do you expect any of the donors to be compatible with Tran Song Lum?

(If you were wondering why Donor 3 is missing it’s because I have already selected 4 donors who are also D negative and compatible with the patient according to their blood group which we tested for in tubes)

 

Answers

ABO + Rh D Patients

  • Tran Song Lum – A Rh D Neg
  • 4360507 – O Rh Pos
  • 4360513 – O Rh Neg
  • 4079107 – O Rh Pos
  • 4360532 – A Rh Pos

Antibody Panel

  • The patient had Anti-E

Phenotyping

  • The patient and all donors except for donor 5 are dCe/dce (or r’r)
  • Donor 5 is dce/dce (or rr)
  • The patient’s Anti-E is a result of an allo-antibody as he does not have the E antigen (as shown in his phenotyping card)
  • Based on all the results, the donors should be compatible with the patient and crossmatch negative when performed by IAT.

 

-medlabgeek

 

 

Transfusion Reaction Investigation Practice

In this post I will be showing the practice cases that we had performed so that we could familiarise ourselves with the procedures on a transfusion reaction investigation in NZ. During our lab, we were given two patients that had shown clinical symptoms during a transfusion reaction, resulting in an investigation to determine if it was due to something serious aka ABO incompatible.

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CAT Practice 5

This semester we are performing our patient and donor blood typing using Ortho Biovue cassettes instead of the Bio-Rad cards. There isn’t much different between the two but in NZ, the usage of these two different companies is quite even so we are learning how to use these this semester so that we are familiar with them during our placements and future work environment.

Though they are quite similar, there are a couple of differences. For example, the size of the cards/cassettes (Biovue is smaller); centrifuge times (Biovue is 5 min and Bio-Rad is 10 min); and the matrix within the cards (Bio-Rad uses a gel whereas Biovue uses beads). The technique behind them is the same even though the matrix is different.

Below are a few examples for you guys to practice ABO typing for donors (notice that these cards only have forward typing columns, so are ONLY suitable for donor typing).

You can always check out some of my other CAT practice posts for more examples – check out my most recent Bio-Rad one here.

As always, the answers will be at the bottom of the post 🙂

-medlabgeek

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Aside

Semester Two Begins

The holidays are well and truly over  😦 Today was our first day back to uni and I felt so unprepared. I haven’t purchased my lab manuals yet (which reminds me I should buy them tomorrow after class).

I was so grateful that I passed all my semester exams and found out about my results before I left for my trip to Queenstown (which you can read all about here) as it let me fully relax and have fun without stressing over all these what if’s. Who else overthinks and stresses over “little things” because they just can’t help it?

It also meant that I don’t have to stay back an extra year or screw up any placement opportunities I might have after this semester. Talking about placements, I had my interview for Lab Plus a week ago and I think it went well buuuuut I won’t know my specific placements until October. Finger’s crossed that I got it! ><

My first class for Med Micro III starts on thursday and I’m so excited because I think we will be learning about fungi and parasites. So I will be writing future blog posts on those specific organisms (hopefully!). Not too sure what I will be learning for Transfusion III but it is bound to be interesting because I LOVE transfusion.

Any who, we were welcomed with lure modules being set in specific pokestops around our campus to celebrate the start of the semester, and I was sure to make the most of that during my lunch break. What kind of “promos” for Pokemon go do you all have going around you? It’s so amazing how big this game got but also suffocating reading all about it everywhere I look (I know I’m doing it right at this minute haha).

 

Hmm… I wonder how long my obsession over this Pokemon game will last….

-medlabgeek

 

 

Human Leukocyte Antigen (HLA) Typing

Hi guys, can you believe it’s almost the middle of the year already?! I feel like I only started this semester a month ago. I know everyone says this or mentions it but WHERE DOES THE TIME GO??

If you can recall from last week, I did a quick little post (read about it here) about how I visited NZBS for HLA typing practice. Well today I will be writing a basic post on the theory behind it.

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20/05/16

Just completed a lab conducted by NZBS staff on HLA typing. Used some very antique and nifty Hamilton Pippettes on Terasaki plates and then went to NZBS to look at the results of our cells fluorescence under the microscope. Was a cool experience getting to do something new. Will do a blog soon about HLA typing, but for now here is a photo I took of the pipette !